برگزاری ژورنال کلاب انجمن بیوتکنولوژی پزشکی

ژورنال کلاب انجمن بیوتکنولوژی پزشکی با عنوان: Using the MCOT I-II cyclotide scaffold to design a stable cyclic peptide antagonist of SET, a protein over expressed in human cancer توسط  منصور پورابراهیم، دانشجوی دکترای بیوتکنولوژی پزشکی، ۲۶ دیماه برگزار شد. خلاصه مطلب ارائه شده به شرح زیر است:   The SET protein is a promising drug target in cancer therapy, because of its ability to inhibit the function of the tumor suppressor gene protein phosphatase 2A (PP2A). COG peptides, derived from apolipoprotein E (apoE), are potent antagonists of SET; they induce cytotoxicity in cancer cells upon binding to intracellular SET and modulate the nuclear factor kappa B (NF-κB) signaling pathway. However, the therapeutic potential of COG peptides is limited, because of their poor proteolytic stability and low bioavailability. In this study, the COG peptide, COG1410, was stabilized by grafting it onto the ultrastable cyclic peptide scaffold, Momordica cochinchinensis trypsin inhibitor-II (MCoTI-II). The grafted MCoTI-II peptides were cytotoxic to a cancer cell line and showed high stability in human serum. The most potent grafted MCoTI-II peptide inhibited lipopolysaccharide (LPS)-mediated activation of NF-κB in murine macrophages. Overall, this study demonstrates the application of the MCoTI-II scaffold for the development of stable peptide drugs for cancer therapy.